The measurement of oxygen consumption of Daphnia by a modification of the Cartesian diver technique.
نویسنده
چکیده
The use of the Cartesian diver as a micromanometer was first described by Linderstrem-Lang (1937). Boell, Needham & Rogers (1939) adapted the method to the measurement of the respiration of fragments of amphibian embryos and, in this and subsequent papers, many details of technique were worked out (see Needham, Rogers & Shen Shih-Chang, 1939). Boell, with other collaborators, has used the technique to measure the respiration of early mammalian embryos (Boell & Nicholas, 1939), and to estimate the distribution of cholinesterase (Boell & Nachmansohn, 1940; Boell & Shen Shih-Chang, 1944). Further use of the technique in Linderstrem-Lang's laboratory has included the measurement of the oxygen consumption of tissue cultures (Zamecnik, 1941). A detailed theoretical analysis of the method has been undertaken (Linderstrom-Lang & Holter, 1942; LinderstremLang, 1943), and based on this further technical details have been worked out (Holter, 1943). In all these methods the total capacity of the divers has been less than 40 /A., and this capacity has been greatly reduced in further modifications of the technique described by Zeuthen (1943, 1946).* In order to adapt the method to the measurement of the oxygen consumption of Daphnia it was found necessary to design divers capable of accommodating 40-50 /A. of fluid in which four to five Daphnia were suspended. Thus it became necessary to increase the total capacity to over 100 [A., creating certain possibilities of error, which have been experimentally investigated, and the results are included in the description of the modified technique.
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عنوان ژورنال:
- The Journal of experimental biology
دوره 25 4 شماره
صفحات -
تاریخ انتشار 1948